Private well bacteria sample
How to take a bacteria sample (DEQ steps)
These steps are from DEQ’s “Sampling Guide for the Collection of SM 9223B Total Coliforms and E. Coli,” the private-well instructions (8999-GUI30-R02-102324). The guide says the drinking water standard in these words: “Drinking Water Standard for Total Coliform is set at zero (<1.0) or absent.” This page quotes that line. It is not medical advice, and it does not promise a drinking-water result.
- Samples must be returned within 28 hours of collection. Do not ship on a Friday or weekend.
- The kit is a chain of custody form and one 100 mL plastic bottle with preservative (a white pill or white powder). A barcode label is included when the lab provides one.
- Remove the aerator. Use only a two-handled faucet, and only the cold side. Do not use a single-handle faucet, an outside tap, or a frost-free hydrant.
- Clean the cold faucet threads, inside and out, with at least a 10% bleach solution.
- Run the cold water for 5 minutes. Then reduce the flow to a slow, steady stream.
- Do not touch the inside of the lid or the bottle. Do not remove the white pill or powder.
- Fill the bottle slightly above the 100 mL line. Do not fill to the top of the neck. Replace the cap tightly, and do not over-tighten it.
- If a barcode is not provided, label the bottle, not the lid, with the date and time (a.m. or p.m.), the collector, and the sample location. Complete the chain of custody. Sign and date it before you mail the sample.
- The lab rejects the sample if the form is incomplete, illegible, or missing; the bottle is not labeled appropriately; the sample is under 100 mL; it is not received within 28 hours; it is not in the DEQ 100 mL bottle; it is frozen; or it is damaged beyond usability.
Courier address on the guide: Oklahoma Department of Environmental Quality, State Environmental Laboratory, 707 N. Robinson, Oklahoma City, OK 73102. For USPS, the same guide says to use P.O. Box 1677, Oklahoma City, OK 73102. For personal delivery, sign the chain of custody at the receiving window of the DEQ Sample Management Unit, through the door on the northeast corner of the building. Hours on the guide are Monday through Friday, 8:00 a.m. to 4:30 p.m., except state holidays.
The guide also says to call 405-702-1000 or email [email protected] for further assistance.